1. 基本信息
品系名称:C57BL/6-Gt(ROSA)26Sortm1(CAG-tdTomato)/Vst
常用名:B6-tdToamto; tdToamto小鼠
背景:C57BL/6NCrl
毛色:黑色
品系建立:
在Rosa26位(wei)点定点敲入CAG-tdToamto序列,获得全身表达红(hong)色荧光的首(shou)建(jian)鼠(shu)。首(shou)建(jian)鼠(shu)与野(ye)生(sheng)型(xing)C57BL/6NCrl交配(pei)获得杂合鼠(shu),杂合鼠(shu)互配(pei)获得纯合鼠(shu)。
2. 表(biao)型
除(chu)红(hong)细(xi)胞和毛发外,该(gai)小鼠品系(xi)的(de)细(xi)胞可以激发出红(hong)色荧(ying)光(tdToamto).
3. 应用(yong)领域(yu)
小(xiao)鼠(shu)细胞(bao)或组织追踪;另外分(fen)离自tdToamto红色(se)荧(ying)光小(xiao)鼠(shu)的细胞(bao)可能(neng)比转染EGFP质粒的野生型小(xiao)鼠(shu)原代细胞(bao)更容易培养。
4. 参考文献
(1)Madisen L; Zwingman TA; Sunkin SM; Oh SW; Zariwala HA; Gu H; Ng LL; Palmiter RD; Hawrylycz MJ; Jones AR; Lein ES; Zeng H. 2010. A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci 13(1):133-40.
(2)Strathdee D, Ibbotson H, Grant SG. Expression of transgenes targeted to the Gt(ROSA)26Sor locus is orientation dependent. PLoS One. 2006 Dec 20;1:e4.