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1. 基本信息
品系(xi)名称(cheng) NOD. Prkdcscid Il2rgtm1 Vst Tg(Csf1p -IL3)Vst/Vst
常用名 hIL-3 NPG /Vst; hIL-3 NPG
背(bei)景 NOD-scid/NcrCrl
毛色 白色
品(pin)系建立：
采用转基因的方法(fa)，构建(jian)pCDNA3.1-Csf1p-IL3载体(ti)，将线(xian)性化Csf1p-IL3表达序列注射到NPG小鼠的原核中，在获(huo)得的后代中筛选到合适表达量的阳性小鼠。
在隔离器中，按照近交的方(fang)式(shi)扩繁(fan)建(jian)立商(shang)品化的hIL-3 NPG小鼠品系。
2. 表型
在(zai)NPG小(xiao)(xiao)鼠中(zhong)(zhong)表达人(ren)(ren)的hIL-3细(xi)(xi)(xi)(xi)胞(bao)因(yin)子(zi)。白细(xi)(xi)(xi)(xi)胞(bao)介素3刺激(ji)多(duo)能造(zao)(zao)血干细(xi)(xi)(xi)(xi)胞(bao)分化(hua)为髓系(xi)祖细(xi)(xi)(xi)(xi)胞(bao)。可以(yi)与表达人(ren)(ren)GM-CSF、M-CSF细(xi)(xi)(xi)(xi)胞(bao)因(yin)子(zi)的NPG小(xiao)(xiao)鼠一(yi)起，在(zai)移(yi)植人(ren)(ren)CD34+造(zao)(zao)血干细(xi)(xi)(xi)(xi)胞(bao)6-8周后，于外周血、骨髓、胸腺和(he)(he)脾脏(zang)以(yi)及(ji)包括肺(fei)和(he)(he)肝(gan)在(zai)内(nei)的非(fei)淋(lin)巴组织中(zhong)(zhong)，形成稳定广泛的髓系(xi)和(he)(he)淋(lin)巴系(xi)细(xi)(xi)(xi)(xi)胞(bao)分化(hua)。在(zai)血液和(he)(he)组织中(zhong)(zhong)，可检测(ce)到粒(li)细(xi)(xi)(xi)(xi)胞(bao)分化(hua)（嗜碱性粒(li)细(xi)(xi)(xi)(xi)胞(bao)、中(zhong)(zhong)性粒(li)细(xi)(xi)(xi)(xi)胞(bao)和(he)(he)肥大细(xi)(xi)(xi)(xi)胞(bao)），抗原呈递(di)细(xi)(xi)(xi)(xi)胞(bao)分化(hua)（树突(tu)状细(xi)(xi)(xi)(xi)胞(bao)和(he)(he)巨噬(shi)细(xi)(xi)(xi)(xi)胞(bao)）和(he)(he)调节性T细(xi)(xi)(xi)(xi)胞(bao)群体等。表达人(ren)(ren)M-CSF、GM-CSF和(he)(he)人(ren)(ren)IL-3细(xi)(xi)(xi)(xi)胞(bao)因(yin)子(zi)与Hu-NPG人(ren)(ren)源化(hua)小(xiao)(xiao)鼠相(xiang)比，人(ren)(ren)类(lei)造(zao)(zao)血干细(xi)(xi)(xi)(xi)胞(bao)（hsc）的整体植入水平更高，分化(hua)细(xi)(xi)(xi)(xi)胞(bao)种类(lei)更多(duo)。
3. 应用领域
-人(ren)源化(hua)和癌(ai)症治(zhi)疗
-人体过敏反应模(mo)型，免疫、造血移植(zhi)重建模(mo)型
-再生医学
-感染性疾病
-生(sheng)医学
4. 参考文献
（1）Ito R, Takahashi T, Katano I, Kawai K, Kamisako T, Ogura T, Ida-Tanaka M, Suemizu H, Nunomura S, Ra C, Mori A, Aiso S, Ito M. (2013) Establishment of a human allergy model using human IL-3/GM-CSF-transgenic NOG mice. J Immunol.191(6):2890-9.
（2）Wunderlich M; Chou FS; Link KA; Mizukawa B; Perry RL; Carroll M; Mulloy JC. 2010. AML xenograft efficiency is significantly improved in NOD/SCID-IL2RG mice constitutively expressing human SCF, GM-CSF and IL-3. Leukemia 24(10):1785-8.
（3）Billerbeck E; Barry WT; Mu K; Dorner M; Rice CM; Ploss A. 2011. Development of human CD4+FoxP3+ regulatory T cells in human stem cell factor-, granulocyte-macrophage colony-stimulating factor-, and interleukin-3-expressing NOD-SCID IL2R{gamma}null humanized mice. Blood 117(11):3076-86.
（4）Coughlan AM; Harmon C; Whelan S; O'Brien EC; O'Reilly VP; Crotty P; Kelly P; Ryan M; Hickey FB; O'Farrelly C; Little MA. 2016. Myeloid Engraftment in Humanized Mice: Impact of Granulocyte-Colony Stimulating Factor Treatment and Transgenic Mouse Strain. Stem Cells Dev 25(7):530-41.
